Goals for Phytobiomes||Technical Needs||
- Define who is present and the relationships of phytobiome composition to plant productivity.
- High throughput community profiling, phenotypic and environmental profiling, and metagenomics analyses
- Efficient approaches for meta-analyses of large numbers of genomic datasets
- Identify characteristics of healthy vs. nonadaptive phytobiomes
- Define core phytobiomes associated with consistent plant productivity, specific to distinct environments
- Determine targets for inoculant development, or for populations to suppress disease
- Identify key drivers of phytobiome composition and dynamics.
- Extensive correlative data and systematic experimental data from diverse habitats and environmental conditions
- Efficient approaches for integration and analysis of complex community data across diverse systems
- Identify potential suite(s) of facotrs that may be targeted by field management
- Determine the functional capacities of individual taxa and simple communities to alter plant productivity.
- High-throughput plant phenotyping, including root systems
- High-throughput, in vivo/in planta microbial functional analyses and transcriptomics
- Identify potential inoculant or inoculant mixtures
- Identify unexpected functional outcomes, and correlates of unexpected outcomes (e.g. taxonomic or functional characteristics of individual taxa or combinations of taxa that lead to better-than-expected, or poorer-than-expected, plant outcomes)
- Characterize the emergent properties of phytobiomes.
- System-biology approaches for analysis and prediction of complex community effects on individual microbial properties/phenotypes and on plant phenotypes
- High-throughput microbial and plant transcriptomics
- Develop core principles for designing optimal synthetic microbiomes for maximizing plant productivity
- Design novel approaches for managing phytobiomes to optimize plant productivity.
- Efficient, reproducible, high-throughput field testing platforms
- Integrate information from goals A-D to propose broad-based management solutions for agriculture
All goals should be accomplished for diverse plant hosts across a broad range of habitats and environmental conditions, and every goal addresses multiple specific questions.